Fig. 8

BBB gene levels after exposure to PX in vivo. (a) Timeline of the experiment. Mice were injected intramuscularly with saline or 0.45 mg\kg PX alone or 0.45 mg\kg PX pre-treated with 100 mg\kg VX-765 which was administrated IP, and their hippocampi were dissected 4 or 24 h later for blood vessels isolation and RNA extraction (all treatments were given for 4 h, unless mentioned differently). Fold change in mRNA expression levels of the indicated genes as determined by NanoString analysis: (b) Adhesion molecules and chemoattractant genes, (c) tight and adherens junction genes, (d) transporters related genes, (e) caspase-1 pathway genes. Fold change of each gene was calculated as the ratio of the average gene expression in the control group to that of the PX-treated groups. Normalized NanoString gene expression data are presented as mean fold change ± SEM. N = 4 mice for each treatment. *p < 0.05, **p < 0.01, ***p < 0.001 vs. control, and #p < 0.05 vs. PX