Fig. 3
miR-155 inhibitor molecule inhibits expression of miR-155 in spleens of Lm infected mice. Male C57BL/6J mice were infected with 2.2–2.3 × 105 CFU Lm EGD, then treated with antibiotics. Uninfected mice (circle) received antibiotics. miR-155 inhibitor (black upward-pointing triangle, black downward-pointing triangle) or LNA scramble (white upward-pointing triangle, white downward-pointing triangle) injected s.c. on 2d, 4d, 6d, and 8d p.i. Mice were sacrificed on 3d, 7d, 14d p.i. Experimental timeline is shown in (a). miR-155 expression in spleens was measured by qPCR and normalized to sno135 (b). Values are expressed in terms of fold change, with uninfected = 1. Significant p values compared with uninfected mice by one-way ANOVA with Dunnett’s T3 multiple comparisons test are indicated (*p < 0.05). Statistical differences between infected mice treated with miR-155 inhibitor or LNA scramble at individual time points calculated via two-tailed Student’s t test (3d) Mann-Whitney U test (d14) are given.