Fig. 1

Voluntary physical exercise reverses cognitive impairment in 5xFAD mice. Nest building, elevated plus-maze, open field, and MWM tests were performed on WT-SED, WT-EXE, 5xFAD-SED, 5xFAD-EXE male mice at 7 months of age to assess effect of voluntary exercise on behavior. a Nest-building rating score. b Percentage of spent time and frequency of entries in open arms of elevated plus-maze test. c Total moved distance, time in the center, frequency of entries in the center, and latency to center entries in open field test. d Evolution of swimming speed and time spent in wall zone during 5-day acquisition trials and distance in platform zone during probe trial in MWM test. All data are presented as mean (±SEM). Two-way ANOVA to measure genotype and exercise effect between WT and 5xFAD mice followed by Mann-Whitney test (a,b) or unpaired t test (c) comparison in case of significant interaction between two factors (genotype*exercise); two-way ANOVA for repeated measures (d), with the test day as the within-subject factor. n = 20-22 per group. «*» Genotype effect, «#» exercise effect: *p < 0.05, **p < 0.01, ***p < 0.001, #p < 0.05, ##p < 0.01, ###p < 0.001