Table 2 Table of antibodies used
Antigen | Host species and clone | Cat. # or Lot # | RRID | Conjugation | Source | Used concentration | Methods |
|---|---|---|---|---|---|---|---|
ASC | Rabbit polyclonal | abx013852 | Â | Â | Abbexa | 1:1000 | WB |
caspase-1 | Rabbit monoclonal | ab179515 | Â | Â | Abcam | 1:1000 | |
β-actin | Rabbit polyclonal | BL005B |  |  | Biosharp | 1:2000 | |
IL-18 | Rabbit polyclonal | PA5-79481 | AB_2746597 | Â | Invitrogen | 1:1000 | |
IL-1β | Rabbit polyclonal | ab9722 |  |  | Abcam | 1:1000 | |
Rabbit IgG (H + L) | Goat polyclonal | BL003A | Â | HRP | Biosharp | 1:10,000 | |
CD11b | Rat monoclonal | 14-0112-82 | AB_467108 | Â | Invitrogen | 1:200 | IHF |
CD45 | Rat monoclonal | 14-0451-82 | AB_467251 | Â | |||
CD68 | Rat monoclonal | MA5-16674 | AB_2538168 | Â | |||
CCR7 | Rabbit polyclonal | ab191575 | Â | Â | Abcam | ||
Arg1 | Rabbit polyclonal | PA5-29645 | AB_2547120 | Â | Invitrogen | ||
CD4 | Rat monoclonal | 14-9766-82 | AB_2573008 | Â | |||
CD4 | Rabbit polyclonal | PA5-87425 | AB_2804136 | Â | |||
GATA3 | Rabbit polyclonal | PA5-20892 | AB_11154392 | Â | |||
T-bet | Rabbit polyclonal | PA5-40573 | AB_2576589 | Â | |||
ROR gamma (t) | Rabbit polyclonal | PA5-23148 | AB_2540675 | Â | |||
FOXP3 | Rat monoclonal | 14-4776-82 | AB_467554 | Â | |||
Rat IgG (H + L) | Goat polyclonal | 112-095-143 | AB_2338199 | Fluorescein (FITC) | Jackson ImmunoResearch | ||
Rabbit IgG (H + L) | Goat polyclonal | 111-025-144 | AB_2337932 | Rhodamine (TRITC) | |||
CCR7 | Rat monoclonal | 47-1971-82 | AB_2573974 | APC-eFluor 780 (AF780) | Invitrogen | 0.25 μg/test | FCM |
IgG2b kappa isotype control | Rat | 47-4321-82 | AB_1271997 | APC-eFluor 780 (AF780) | 0.25 μg/test | ||
CD11b | Rat monoclonal | 12-0112-81 | AB_465546 | PE | 0.125 μg/test | ||
CD3 | Rat monoclonal | 12-0032-82 | AB_2811741 | PE | 0.25 μg/test | ||
IgG2b kappa isotype control | Rat | 12-4031-82 | AB_470042 | PE | 0.25 μg/test | ||
CD127 | Rat monoclonal | 48-1273-82 | AB_2574039 | eFlour 450 | 0.5 μg/test | ||
IgG2b kappa isotype control | Rat | 48-4031-82 | AB_1272017 | eFlour 450 | 0.5 μg/test | ||
CD183 | Armenian hamster monoclonal | 62-1831-82 | AB_2762747 | Super Bright 436 (SB436) | 0.5 μg/test | ||
IgG isotype control | Armenian Hamster | 62-4888-82 | AB_2717007 | Super Bright 436 (SB436) | 0.5 μg/test | ||
CD196 | Rat monoclonal | 50-7196-82 | AB_11219682 | eFlour 660 | 0.5 μg/test | ||
IgG2a kappa isotype control | Rat | 50-4321-82 | AB_10598503 | eFlour 660 | 0.5 μg/test | ||
CD25 | Rat monoclonal | 47-0251-82 | AB_1272179 | APC-eFluor 780 (AF780) | 0.5 μg/test | ||
IgG1 kappa isotype control | Rat | 47-4301-80 | AB_1271986 | APC-eFluor 780 (AF780) | 0.5 μg/test | ||
CD28 | Syrian hamster monoclonal | 45-0281-80 | AB_925744 | PerCP-Cyanine5.5 (PE-cy5.5) | 0.5 μg/test | ||
IgG isotype control | Armenian hamster | 45-4888-80 | AB_906260 | PerCP-Cyanine5.5 (PE-cy5.5) | 0.5 μg/test | ||
CD3 | Armenian hamster monoclonal | 47-0031-82 | AB_11149861 | APC-eFluor 780 (AF780) | 0.5 μg/test | ||
IgG isotype control | Armenian hamster | 47-4888-80 | AB_1271978 | APC-eFluor 780 (AF780) | 0.5 μg/test | ||
CD4 | Rat monoclonal | 11-0041-82 | AB_464892 | FITC | 0.25 μg/test | ||
CD68 | Rat monoclonal | MA5-16676 | AB_2538170 | FITC | 0.25 μg/test | ||
IgG2b kappa isotype control | Rat | 11-4031-82 | AB_470004 | FITC | 0.25 μg/test | ||
CD45 | Rat monoclonal | 17-0451-82 | AB_469392 | APC | 0.125 μg/test | ||
IgG2b kappa isotype control | Rat | 17-4031-82 | AB_470176 | APC | 0.125 μg/test |