Fig. 2

mRNA expression of inflammatory markers in hippocampi of IL-33-injected mice. mRNA expression of pro-inflammatory markers (Nos2 in a, IL1b in b, Tnfa in c and Ifng in d) or anti-inflammatory markers (Arg1 in e, Chil3 in f, IL10 in g, and Igf1 in h) were quantified in hippocampi at 24 h and 48 h post-surgery by RT-qPCR normalized against 18S RNA. Relative fold change in vehicle group (grey bar) and in rmIL-33 group (black bar) were quantified versus sham group (S; white bar). Minocycline-treated mice were also analyzed at 48 h post-surgery (dotted bar). IL-33 injection delayed the resolution of inflammation highlighted by an increase of inflammatory markers at 48 h administration. A partial reduction of this effect was observed under minocycline exposure, especially for IL-1b and Ifng mRNA. Data are represented as mean ± SEM (n = 4–6). Statistical comparisons were made using Kruskal-Wallis followed by Dunn’s multiple comparison test for each group vs. Sham. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. In addition, a comparison between rmIL-33 (48 h) and rmIL-33 + mino (48 h) groups was performed using the Mann-Whitney test. #P ≤ 0.05, ##P ≤ 0.01