Fig. 5

Increased IL-1β expressing microglia in the hippocampus of IL-33-treated mice. Flow cytometry revealing an increased proportion of microglia associated with a IL-1β pro-form overexpression. a Gating strategies to analyze whole hippocampus cell suspensions. A gate was created on non-debris population excluding very low SSC/FSC events. Then, cells were gated on single cells and selected on their live dead staining. This population was then gated according to CD11b and CD45 status as populations of CD11b^low/CD45^high lymphocytes (b), CD11b^high/CD45^high macrophages (c) and CD11b^high/CD45^low microglia (d). The percentage of lymphocytes, macrophages, and microglia obtained from two experiments (n = 4–5 by the group) are graphically reported. e Representative geometric mean fluorescent intensity (GMFI) curve of IL-1β proform expression in microglial gated cells. f Microglial cells MFI for pro-IL-1β immunostaining expressed as ratio control relative to sham. Our flow cytometry analysis shows that compared with sham controls, rmIL-33 treatment induces a higher proportion of microglial cells correlated with a higher expression of IL-1β pro-form. Data are shown as mean ± SEM. Statistical analyses were made by Kruskal-Wallis test, followed by corrected Dunn’s multiple comparison test. *P ≤ 0.05, **P ≤ 0.01