Fig. 3
Lower doses of NE increase neutrophil population among splenocytes. a Schematic representation of in vitro assay and flow cytometry assessment. b Flow cytometry plots demonstrating monocytes/macrophages (SSClowLy6G−) and neutrophil (SSChighLy6G+)populations. c, d Frequency and concentration of neutrophils after NE stimulation. e, f Frequency and concentrations of monocytes/macrophages after NE stimulation. N (0 M) = 4; n (1 × 10−9) = 5; n (1 × 10−6) = 5; n (1 × 10−3) = 5. Data representative of three independent experiments. Statistical tests: b–f one-way ANOVA; post hoc Tukey’s multiple comparisons test. Results expressed as mean ± SEM. n.s. non-statistically significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001