Skip to main content
Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Cottonseed oil alleviates ischemic stroke injury by inhibiting the inflammatory activation of microglia and astrocyte

Fig. 7

CSO treatment affected the activation of A1/A2 type reactive astrocytes in the ischemic penumbra. A CSO treatment decreased the number of A1 type astrocytes and increased the number of A2 type astrocytes. A(a) Representative immunofluorescence images showing co-localization of the astrocyte labeled with GFAP and A1 astrocyte labeled with C3d, and co-localization of the astrocyte labeled with GFAP and A2 astrocyte labeled with S100A10 in the ischemic penumbra 24 h after reperfusion. Scale bar in the Sham-Con and Sham-CSO groups = 50 μm. Scale bar in the MCAO-Con and MCAO-CSO groups = 20 μm. A(b) Percentage of C3d-positive cells that are GFAP-positive in the ischemic penumbra. ##p < 0.01 vs. Sham-Con group, *p < 0.05 vs. MCAO-Con group. n = 5 per group. (c) Percentage of S100A10-positive cells that are GFAP-positive in the ischemic penumbra. ##p < 0.01 vs. Sham-Con group, *p < 0.05 vs. MCAO-Con group. n = 5 per group. B CSO treatment decreased C3d protein and increased S100A10 protein expression in the ischemic penumbra 24 h after reperfusion. B(a) Cropped gels and blots showing the protein expression of the C3d and S100A10. B(b) Graph of the protein expression of the C3d and S100A10. ##p < 0.01 vs. Sham-Con group, *p < 0.05 vs. MCAO-Con group. n = 6 per group

Back to article page