Fig. 4

Neuronal density detection in cortex (a, b, c, and d, respectively), thalamus (e, f, g, and h, respectively), cerebellum (i, j, k, and l, respectively), and pons (m, n, o, and p, respectively) of 4.5-month-old WT, Hexa−/−, Neu3−/−, and Hexa−/−Neu3−/− mice. They were stained with anti-NeuN antibody (red) and DAPI (blue). The histograms represent quantification of neuronal density in the cortex (R), thalamus (S), cerebellum (T), pons (U). Scale bar = 50 μm for cortex and thalamus; 100 μm for cerebellum and pons. The data are represented as the mean ± SEM. One-way ANOVA was used for statistical analysis (*p < 0.05, **p < 0.025, ***p < 0.01, and ****p < 0.001)