Fig. 3
From: β-Hydroxybutyrate inhibits inflammasome activation to attenuate Alzheimer’s disease pathology
BHB treatment results in less pronounced microgliosis in 5XFAD mice. Brain sections were prepared as in Fig. 2. The number of microglia/field (a) and the number of microglia within 15 μm of plaque surfaces (b) were determined using Imaris 9.2.1. (c) The box-counting method was used to calculate the fractal dimension of outlined versions of non-plaque-associated microglia (n = 3/image; 9 microglia/mouse) using FracLac for ImageJ. Microglial cells were outlined and ordered according to least complex (left) to most complex (right). (d) Cumulative distribution of fractal dimensions of non-plaque-associated microglia. Graphs show data as the mean ± SD with ****P ≤ 0.0001, ***P ≤ 0.001, **P ≤ 0.01, and N.S. = not significant