Fig. 2

Langat virus infection leads to asymptomatic infection in wildtype mice and brain region specific replication. Wildtype (n = 8) and Irf-7^−/− mice (n = 10) were intraperitoneally infected with 10⁴ FFU of LGTV. a Body weight. Data represent the mean with SEM of n ≥ 8 mice per group and time point. Quantitative RT-PCR was performed for the olfactory bulb (OB) and the hippocampus (HC). Results were normalized to β-actin for b LGTV, and c IL-6 and TNF-α. The dotted line represents the linear detection limit. Data represent the mean with individual values of n ≥ 5 per group and time point. For flow cytometry analysis of the brain, percentage of infiltrating cells (CD45⁺) (d) and microglia (CD45^lo/intCD11b⁺) (e). f Representative dot blots of CD45 and CD11b staining are depicted: microglia (CD CD45^lo/intCD11b⁺, infiltrating cells (CD45⁺)). g Immunhistological analysis of the olfactory bulb 16 days post-infection. DAPI (blue) and LGTV E-protein (green). Magnification × 40. Data were analyzed by two-way ANOVA with Tukey’s multiple comparison tests. *p < 0.05, **p < 0.01, ****p < 0.0001