Fig. 7

Immunohistochemical analyses of cell adhesion and extracellular matrix DEGs. a Fluorescence images showing cross-sections of unoperated control nerve, 14 days PO PEG-fused PNA, and 14 days PO NC PNA immunostained for COL1A1 (green). b Percent (%) COL1A1 staining area comparisons at 14 days PO (n = 4 per treatment group). c Normalized read counts of COL1A1 RNA transcripts at 14 days PO. Fluorescence images showing immunostaining for CD24 (red) in cross-sections. d Fluorescence images showing immunostaining for CD24 (red). e Percent (%) CD24 staining area comparisons at 14 days PO (n = 4 per treatment group). f Normalized read counts of CD24 RNA transcripts at 14 days PO. Fluorescence images showing immunostaining for CD24 (red) in cross-sections. g Fluorescence images showing immunostaining for THBS1 (red). h Percent (%) THBS1 staining area comparisons at 14 days PO (n = 4 per treatment group). i Normalized read counts of THBS1 RNA transcripts at 14 days PO. j Fluorescence images showing immunostaining for THBS2 (red). k Percent (%) THBS2 staining area comparisons at 14 days PO (n = 4 per treatment group). l Normalized read counts of THBS2 RNA transcripts at 14 days PO. m Fluorescence images showing immunostaining for CD276 (red). n Percent (%) CD276 staining area comparisons at 14 days PO (n = 4 per treatment group). o Normalized read counts of CD276 RNA transcripts at 14 days PO. Nuclei are stained with DAPI (blue). Scale = 50 μm. In graphs, data are shown as mean (horizontal bar) with each individual data point per animal. In b, e, h, k, and n, overall one-way ANOVA p values are shown above brackets at each time point, with multiple comparisons p values between treatment groups displayed underneath