Fig. 3
Enhanced BBB leakage in PI3Kγ-deficient mice at reduced Ta 24 h after LPS-induced SIRS. a Mice kept under neutral Ta show mild disturbance of BBB integrity in PI3Kγ mutant. LPS-induced SIRS elicits small increase of BBB leakage in wild-type mice (Wt, open boxplots). b Reduced Ta was accompanied by enhanced Evans blue (EB) extravasation into brain tissue indicating degree of BBB leakage in PI3Kγ-deficient (PI3Kγ−/−) mice (filled columns) in comparison with Wt and kinase-dead (PI3KγKD/KD, hatched boxplots) mice. c Representative pictures of native brain slices immediately after transcardial rinsing with physiological saline, brain removal, and cutting into coronal slices using a brain matrix. Values are presented as boxplots illustrating medians within boxes from first quartile (25th percentile) to the third quartile (75th percentile) and whiskers ranging from minimum to maximum, n = 10 per group and time point. * † § p < 0.05, asterisk indicates significant differences versus baseline conditions within the same genotype, dagger sign indicates significant differences versus neutral Ta within the same genotype, section sign indicates significant differences versus Wt mice within the same Ta condition (two-way ANOVA and one-way ANOVA, followed by Holm–Sidak test for post hoc multiple comparisons was performed for comparison between respected groups, t test was used for comparisons between states within same groups with Bonferroni’s correction for adjustments of multiple comparisons)