Fig. 5

Hypothalamic microgliosis in AD mice occurs in a nucleus-specific manner but is unaffected by sex or HF diet. Labeling of tissue sections including the hypothalamus was performed for Iba1. Quantification was performed for percent area positive for each label in the arcuate nucleus (a), ventromedial nucleus (b), dorsomedial nucleus (c), paraventricular nucleus (d), and lateral hypothalamus area (e). Representative images for labeling with Iba1 are also shown (f). N = 3–5/group. ^ = AD effect p < 0.05; ^^ = AD effect p < 0.01; ^^^ = AD effect p < 0.001