Fig. 8

TLR2 is necessary for HK Lb-mediated protection in EAE. a Concentration of TLR2 ligand in the plasma of mice treated with PBS or HK Lb at 0 DPI and 14 DPI (n = at least 4 mice per group). b EAE clinical scores and c incidence for wild-type and TLR2-deficient C57BL/6 mice treated with PBS or HK Lb (n = at least 21 mice per group). d Quantification of demyelination by LFB staining in the spinal cord cross-sections at 30 DPI (n = at least 5 mice per group and at least 6 sections per mouse). e Representative LFB staining of the thoracic spinal cord sections from treated mice. f EAE clinical scores and g incidence for TLR9-deficient C57BL/6 mice treated with PBS or HK Lb (n = at least 14 mice per group). Data are pooled from two independent experiments and presented as mean ± SEM. *P ≤ 0.05, **P ≤ 0.01. ns, not significant; Student t test for a, AUC one-way ANOVA with Tukey’s multiple comparisons test for b, log-rank test for c and g, one-way ANOVA with Tukey’s multiple comparisons test for d, and AUC Student t test for f