Fig. 2

EAE-associated miRNAs are expressed in OLs. a–c The number of cells expressing different OL developmental markers was assessed by immunofluorescence in primary mouse OPC cultures. OPCs were fixed with PFA after 0, 3, and 6 days of in vitro differentiation and stained for the OPC marker PDGFRα (in red), the immature OL marker CNPase (in red), or the myelinating OL marker MBP (in green). Nuclei were counterstained with DAPI (in blue). The percentages of cells positive and negative to each marker are plotted. Statistical significance was evaluated by Fisher’s exact test on the total number of cells from two independent cultures. Scale bar: 50 μm. d Expression analysis by qRT-PCR of the 20 miRNAs identified by miRAP in OPCs incubated in differentiation medium for 0, 3, or 6 days. Expression levels are shown as fold differences (mean ± SEM) compared to day 0 (n = 3 cultures). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001