Fig. 3

OPC maturation is controlled by specific EAE-associated miRNAs. a OPCs overexpressing mimics of the 20 EAE-associated miRNAs or a negative control (miR-67 from C. elegans) were differentiated for 3 days and the numbers of MBP⁺ OLs were assessed by immunofluorescence microscopy (green). Nuclei were counterstained with DAPI (blue). Representative images for the significant miRNAs are shown. Scale bar: 50 μm. b The percentages of MBP⁺ and MBP^- cells for each condition are plotted. Statistical significance was evaluated by Fisher’s exact test on the total number of cells from two independent transfections. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001