Fig. 4

The effects of EAE-associated miRNAs are mediated by specific pathways. a, b Unsupervised hierarchical clustering of differentially expressed genes (DEGs, fold change ± 1.5) in differentiated OPCs overexpressing miR-190a-5p or miR-6983-5p as compared to control cells transfected with miR-67 mimic. The distances between samples were calculated using the Euclidean distance method (n = 3 transfections). c, d Histograms showing the most significant KEGG pathways for the DEGs associated to miR-190a-5p or miR-6983-5p overexpression. In this analysis, only the nominally significant down-regulated genes were used (P < 0.05), in order to isolate the core functions controlled by each miRNA in differentiated OPCs