Fig. 2

A schematic illustration of CLP procedure and i.c.v. injection. a CLP procedure. The basis of the cecum is indicated by the yellow line. The cecum is ligated (indicated by dotted green line) at half the distance between distal pole and the base of the cecum. b Injection site of the mouse lateral ventricle. The injection site is at 1.0 mm lateral and 0.5 mm posterior to the bregma over the left hemisphere. c A 27 G needle with a 10-μl syringe is used for the injection into the lateral ventricle 2.5-mm deep, perpendicular to the skull surface. Abbreviations: CLP, cecal ligation and perforation; i.c.v., intracerebroventricular