Neuroprotective effects of verbascoside against Alzheimer’s disease via the relief of endoplasmic reticulum stress in Aβ-exposed U251 cells and APP/PS1 mice

Table 1 Details of antibodies used in immunohistochemical and western blot

Number	Antibodies	Molecular weight	Catalog number	Dilution	Application	Company	Area
1	Aβ_1-42		bs-0107R	1:800	Immunohistochemical	Bioss	Beijing, China
2	4-HNE		ab46545	1:200	Immunohistochemical	Abcam	Cambridge, MA, USA
3	tau (phosphor S396)		ab109390	1:4000	Immunohistochemical	Abcam	Cambridge, MA, USA
4	MANF		ab67271	1:400	Immunohistochemical	Abcam	Cambridge, MA, USA
5	MANF	20 kDa	ab67271	1:1000	Western blot	Abcam	Cambridge, MA, USA
6	BiP	78 kDa	ab21685	1:250	Western blot	Abcam	Cambridge, MA, USA
7	p-IRE1 phosphor S724	110 kDa	ab48187	1:1000	Western blot	Abcam	Cambridge, MA, USA
8	CHOP	27 kDa	ab11419	1:1000	Western blot	Abcam	Cambridge, MA, USA
9	IRE1	105 kDa	BS-8680R	1:1000	Western blot	Bioss	Beijing, China
10	XBP1	40 kDa	bs-1668R	1:1000	Western blot	Bioss	Beijing, China
	PERK	122 kDa	BSM-51385M	1:1000	Western blot	Bioss	Beijing, China
11	ATF6	75 kDa	bs-1634R	1:500	Western blot	Bioss	Beijing, China
12	ATF4	38 kDa	bs-1531R	1:5000	Western blot	Bioss	Beijing, China
13	p-PERK (Thr982)	125 kDa	DF7576	1:800	Western blot	Affinity Biosciences	Cincinnati, OH, USA
14	eIF2α	38 kDa	9722S	1:1000	Western blot	Cell Signaling Technology	Beverly, MA, USA
15	p-eIF2α (Ser51)	38 kDa	9721S	1:1000	Western blot	Cell Signaling Technology	Beverly, MA, USA
16	caspase12	38 kDa	A0217	1:1000	Western blot	ABclonal	Wuhan, China
17	GAPDH	37 kDa	E-AB-20059	1:2000	Western blot	Elabscience	Wuhan, China
18	Goat anti-mouse		E-AB-1001	1:5000	Western blot	Elabscience	Wuhan, China
19	Goat anti-rabbit		E-AB-1003	1:5000	Western blot	Elabscience	Wuhan, China

ISSN: 1742-2094