Fig. 1

Binding of Ab-T1 to TREM2. a Binding of the mouse IgG antibodies Ab-T1, Ab-T2, Ab-T3, Ab-T4, Ab-T5, and control anti human TREM2 Ab to human and mouse TREM2 expressed in HEK293T cells by dot blot assay. Control naïve HEK293T cells (control) used as negative control. Binding affinity sensograms of Ab-T1 to (b) human TREM2 and (c) mouse TREM2 as measured by surface plasmon resonance (BiaCore). d Ab-T1 binding affinity table; “Ka” refers to the association rate constant; “kd” refers to the dissociation rate constant; and “KD” refers to the affinity constant. e Confocal images of Ab-T1 immunostaining non-permeabilised HEK293T transfected with wild type hTREM2-GFP (upper panels) and parental HEK293T (lower panels). Nuclei stained with DAPI. f Human TREM2 expression levels on transfected HEK293T cells recognized by Ab-T1. The y-axis represents the relative mean fluorescence intensity (relative MFI) measured by flow cytometry. g Western blot showing human TREM2 detection on stable U937 cell line recognized by Ab-T1