Fig. 6

RAM-589.555 does not modulate the morphological shaping of activated microglia and astrocytes. LPS-activated microglia and TNFa-activated astrocytes were cultured in the absence or presence of RAM-589.555 at 50 and 100 nM for 48 h. a Bright field phase contrast images show microglia (upper panel) and astrocytes (lower panel). Scale bar = 50 μm. b Immunofluorescence microscopy images show CD11b⁺ microglia and GFAP⁺ astrocytes stained with DAPI as a marker for cell viability. Images are representative of three independent experimental repeats (4 images per condition). Scale bar = 20μm