Fig. 2

Effects of endothelin administration on the expression of inhibitory and co-stimulatory molecules on monocytes (CD11b⁺) in the CNS of infected mice. a Flow cytometric analyses of CNS-infiltrating cells at 8 dpi. A representative set of plots is shown. CD11c⁺ cells between control vs. ET-1 groups of 3–5 experiments, 2.23 ± 0.18 vs. 4.4 ± 0.11; NK1.1⁺ cells, 2.6 ± 0.25 vs. 5.0 ± 0.37; Ly6G/6C⁺ cells, 13.49 ± 1.00 vs. 19.2 ± 1.69; CD11b⁺CD45^int (microglia), 37.20 ± 1.93 vs. 26.46 ± 2.39; CD11b⁺CD45^hi (macrophage), 2.65 ± 0.45 vs. 5.55 ± 0.49, respectively. Mean ± SD is shown for each cell type. b Cell numbers of the above cell types in the CNS at 8 dpi, as determined by flow cytometry, are shown in the histogram. Differences in the cell numbers of the cell types except the microglia are significant. Data are presented as mean ± SEM of three independent experiments. **p < 0.001 and ***p < 0.0001. c Expression levels of inhibitory (PDL-1) and co-stimulatory molecules (CD40, CD80, CD86, and MHC) on the CNS CD11b⁺ cells in TMEV-infected mice treated with either PBS or ET-1 at 8 dpi (n = 3). The number indicated the % of marker expressed cells of total CD11b⁺ cell. Data are representative of three independent experiments