Fig. 6

Effect of endothelin-1 on PLP-induced EAE. a SJL/J mice (n = 5/group) were immunized with a suboptimal dose (10 μg) of PLP and were injected with PBS or endothelin-1 at 20 μg/kg at −3, 0, 5, 10, 15, 20, and 46 dpi. p < 0.001 between control and ET-1 treated group based on two-tailed paired t test between days 31 and 87 post-immunization. b Levels of CD4, CD8, and cytokine mRNA in the CNS of treated mice. c Proliferative responses and IFN-γ production by splenic T cells from control and ET-1 treated mice (n = 3) to PLP (10 μM) at 20 days after PLP-immunization. Data are presented as mean ± SEM of three independent experiments. **p < 0.001, and ***p < 0.0001