Fig. 2From: Sestrin2 regulates microglia polarization through mTOR-mediated autophagic flux to attenuate inflammation during experimental brain ischemiaSestrin2 reduced cell apoptosis after tMCAO. a–c Western blot and quantification analysis show the expression of bax, bcl2, and cleaved caspase3 in Sham, NS, and Sesn group at 3 days after tMCAO. C-cas3 = cleaved caspase3. N = 4–5 per group. Data are presented as mean ± SEM, *p < 0.05, NS vs. Sesn. d, e TUNEL staining and bar graph show the number of TUNEL+ (red) cells in NS and Sesn groups 1 day after tMCAO. White arrows point at TUNEL+/DAPI+ cells. Scale bar = 100 μm. N = 5 per group. Data are presented as mean ± SEM, *p < 0.05, NS vs. Sesn. f Representative photos of TUNEL+/Neun+ cells in NS and Sesn groups. White arrows point at TUNEL+/Neun+ cells. Scale bar = 50 μmBack to article page