Fig. 1

The expression of neuronal LDLR is decreased following cerebral I/R. a Representative immunofluorescence images of LDLR were co-stained with NeuN (neuron marker). LDLR positive neurons declined in ischemic penumbra. Insets show a higher magnification view. n = 3 in each group. b, c Double immunostaining of LDLR with GFAP (astrocyte marker) or Iba-1 (microglia marker) in the cortex of sham and MCAO mice revealed a modest co-localization. Insets show a higher magnification view. n = 3 for each group. d The expression and time course of LDLR in ischemic penumbra, n = 7 for all groups. e Immunoblotting analysis and quantification of LDLR in OGD-treated neurons, n = 5. Data are shown as mean ± SEM. ^***P < 0.001 versus sham mice. ^##P < 0.01, ^###P < 0.001 versus control neurons. Ctrl, control. Scale bar = 20 μm