Fig. 6

Effects of I3C in retinal microglia reactivity elicited by light in BALB/cJ mice. a-g Representative photomicrographs of IBA 1 stained microglia (green) of retinal flat mounts. Microglia in the subretinal space (SR) and outer plexiform layer (OPL) of control a, e, vehicle + light b, f, and I3C + light c, g treated mice. d Total IBA 1+ cells were counted in the subretinal space (untreated n = 20 retinas, vehicle + light = 20 retinas, and I3C + light n = 20 retinas). h-j Representative photomicrographs show retinal sections of control h, vehicle + light i, and I3C + light j treated mice stained with IBA 1 (green) and DAPI (blue). k For quantification of ONL thickness in cryosections, rows of photoreceptor nuclei were counted (untreated n = 10 retinas, vehicle + light n = 10 retinas, I3C + light n = 10 retinas). Data show mean ± SEM with ****p < 0.0001. ONL outer nuclear layer, OPL outer plexiform layer, INL inner nuclear layer, IPL inner plexiform layer, GCL ganglion cell layer