Fig. 3

The molecular features of senescence in irradiated microglia. a To identify the mRNAs whose expression is altered in irradiated microglia, an RNA-sequencing analysis (RNA-seq) in BV2 cells was performed. BV2 cells were collected 24 h after exposure to 10 Gy using a linac. Pathway analysis (KEGG) of the differentially expressed genes (FDR q value <0.05) identified the first 20 pathways that exhibited significant differences. The pathways in red represent those related to inflammation. b Biological network analysis using CentiScaPe of IR-induced differentially expressed genes (FDR q value <0.05). The size of the nodes is proportional to the gene number, and a darker colored node represents more significant enrichment. c KEGG analysis of 316 significantly changed metabolism-related genes (FDR q value <0.05) identified the top 25 pathways that exhibited significant differences. Hashes (#) represent neurodegenerative diseases. d Network of gene sets related to neurodegeneration in the irradiated microglia gene signature. e-g Expression levels of genes related to inflammation, the DDR, and metabolism in BV2 cells, as examined using qRT-PCR at 24 h after 10 Gy by linac (mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001)