Fig. 5
Transcriptional profiling of Ifnar1 sufficient and deficient microglia exposed to mIFNα. a Volcano plot showing highlights of differentially expressed (DE) genes from bulk RNA sequencing of sorted cortical microglia, comparing vehicle-treated control (N = 6) and tamoxifen-treated knockdown (N = 6) groups from Cx3cr1-CreERT2+/−, Ifnar1fl/fl mice. Interferon-stimulated genes were identified based on querying the Interferome 2.0 database [43] with the DE gene list. q, Benjamini-Hochberg adjusted p value. b Overlap of independently identified > 1.5-fold change DE genes from both sequencing libraries, identified using the glmTreat function in the EdgeR package [58]. Venn diagram sizes are proportional to the numerical values in each category. c Overlap of independently identified gene ontology pathways. Venn diagram sizes are proportional to the numerical values in each category. d A selection of differential gene ontology pathways identified using gProfiler2 [31] enriched in mIFNα exposed Ifnar1 sufficient and deficient microglia