Fig. 6

Ac2-26 protective effects are mediated via FPR2. a Detection of neutrophil granulocytes by naphthol AS-D chloroacetate esterase (NCAE) reaction in the third ventricle demonstrates less severe neutrophil infiltration in Ac2-26-treated Fpr1^−/−, but not Fpr2^−/− mice 28 h post-infection. The figures show representative results from one of five to seven mice per group. b Quantification of neutrophil granulocyte infiltration using neutrophils score (see “Methods” section for detail; one-way ANOVA followed by Bonferroni test; **p < 0.01). c Coronal brain sections of Fpr1^−/− or Fpr2^−/− mice were stained with antibodies against neutrophil granulocytes 28 h after infection with Streptococcus pneumoniae, with or without Ac2-26 i.p. injection (scale bar = 100 μm). d Densitometric quantification of neutrophil staining was assessed from five mice per experimental group (t test; *p < 0.05)