Fig. 1

TonEBP in LPS-induced inflammation in BV2 cells. BV2 cells were stably transfected using lentivirus containing empty shRNA (open bars in b–e) or TonEBP targeting shRNA (solid bars in b–e) as indicated. a The cells were then treated for 24 h with 0 to 100 ng/ml of LPS as indicated and immunoblotted for TonEBP, iNOS, COX-2, and β-actin as shown in left. Right panels show band intensity for TonEBP, iNOS, and COX-2 corrected by intensity of corresponding β-actin band. b Nitrite was measured in the media of cells treated for 24 h with 0 (CTL:1x PBS) or 10 ng/ml LPS using Griess reagent. c RT-qPCR was performed on cells treated for 1 h (for TNF-α and IL-1β) or 6 h (for iNOS, COX-2, and IL-6) with LPS as in b. d TNF-α was measured in the medium after the cells were treated for 6 h with LPS. e The stably transfected cells were treated for 24 h with 0 (CTL:1x PBS) or 100 ng/ml LPS to obtain conditioned media. HT22 cells grown in regular medium were switched to the conditioned media. After 24 h, cell viability was measured using MTT assay. Mean + SD, n = 3. *P < 0.05 compared to empty shRNA. #P < 0.05 compared to CTL