Fig. 2

Cerulenin inhibits LPS-induced inflammation in BV2 cells. a The cells were pretreated for 1 h with 10 μM cerulenin (solid bars in b–d) or vehicle (water) (open bars in b–d), followed by a 24 h treatment with 0 (CTL:1x PBS) or 10 ng/ml of LPS. Immunoblotting and quantitation were performed as in Fig. 1. b Nitrite was measured from the media of cells treated as above except that LPS treatment was for 24 h. c RT-qPCR was performed on cells treated as in b except that LPS was treated for 1 h (for TNF-α and IL-1β) or 6 h (for iNOS, COX-2, and IL-6). d TNF-α was measured in the medium after the cells were treated as in b for 6 h with LPS. Mean + SD, n = 3. *P < 0.05 compared to vehicle. #P < 0.05 compared to CTL