Fig. 2
MRS5980 reduces the level of brain infarction following CCI TBI. a In serial coronal brain sections (2 mm thick) from sham group (side of sham surgery indicated by gray arrows), the level of TTC staining (red) was strong and uniformed indicating high metabolic and adequately perfused mouse brain. In contrast, the level of TTC staining in the brains from mice with TBI (side of CCI indicated by yellow arrows) and treated with vehicle (24 h after CCI TBI) was reduced overall and non-uniform, indicating increased brain infarction (white unstained regions). In brains from mice with CCI TBI (side of CCI indicated by yellow arrow) and treated with MRS5980 (1 mg/kg; i.p.), the level of TTC staining was greater and the regions of infarction (white unstained regions) were less than in untreated mice with TBI. b, c Morphometric analyses of infarct area and volume in TTC-stained brain slices. ND = not detectable. Data are individual value (b) or mean ± SEM (c) of 10 mice/group and analyzed by two-tailed, (b) two-way ANOVA [F(6, 81) = 2908; p = 2.32 × 10−92, η2p = 0.995] with Bonferroni comparisons or (c) one-way ANOVA [F(2, 27) = 5006; p = 2.00 × 10−35, η2 = 0.997] with Dunnett’s comparisons. *p < 0.05 vs. sham and †p < 0.05 vs. TBI + Veh