Table 1 Primary and secondary antibodies used for immunofluorescence
From: Minocycline reduces inflammatory response and cell death in a S100B retina degeneration model
Primary antibodies | Secondary antibodies | ||||
|---|---|---|---|---|---|
Antibody | Company | Dilution | Antibody | Company | Dilution |
Retinal cross-sections | |||||
Brn-3a | Santa Cruz | 1:100 | Donkey anti-goat Alexa Fluor 488 | Abcam | 1:400 |
Cleaved caspase 3 | Sigma-Aldrich | 1:300 | Donkey anti-rabbit Alexa Fluor 555 | Invitrogen | 1:400 |
ED1 | Millipore | 1:250 | Goat anti-mouse Alexa Fluor 488 | Invitrogen | 1:600 |
GFAP | Millipore | 1:400 | Donkey anti-chicken Cy3 | Millipore | 1:500 |
Iba1 | Wako | 1:500 | Goat anti-rabbit IgG Cy3 | Linaris | 1:300 |
PKM | Abcam | 1:500 | Donkey anti-rabbit Alexa Fluor 555 | Invitrogen | 1:400 |
RBPMS | Millipore | 1:300 | Goat anti-rabbit Alexa Fluor 488 | Millipore | 1:500 |
S100B | Novus Biologicals | 1:100 | Goat anti-rabbit Alexa Fluor 488 | Millipore | 1:500 |
Tmem119 | Synaptic System | 1:500 | Goat anti-mouse Alexa Fluor 488 | Invitrogen | 1:600 |
Longitudinal optic nerve sections | |||||
Cleaved caspase 3 | Sigma-Aldrich | 1:100 | Donkey anti-rabbit Alexa Fluor 555 | Invitrogen | 1:400 |
ED1 | Millipore | 1:200 | Goat anti-mouse Alexa Fluor 555 | Invitrogen | 1:500 |
GFAP | Millipore | 1:400 | Donkey anti-chicken Cy3 | Millipore | 1:500 |
Iba1 | Wako | 1:400 | Goat anti-rabbit Alexa Fluor 488 | Invitrogen | 1:500 |
S100B | Novus Biologicals | 1:100 | Donkey anti-rabbit Alexa Fluor 555 | Invitrogen | 1:400 |
SMI-32 | Biolegend | 1:2000 | Goat anti-mouse Alexa Fluor 555 | Invitrogen | 1:500 |
Tmem119 | Synaptic System | 1:500 | Goat anti-mouse Alexa Fluor 488 | Invitrogen | 1:600 |