Skip to main content

Table 1 Primary and secondary antibodies used for immunofluorescence

From: Minocycline reduces inflammatory response and cell death in a S100B retina degeneration model

Primary antibodies Secondary antibodies
Antibody Company Dilution Antibody Company Dilution
Retinal cross-sections
Brn-3a Santa Cruz 1:100 Donkey anti-goat Alexa Fluor 488 Abcam 1:400
Cleaved caspase 3 Sigma-Aldrich 1:300 Donkey anti-rabbit Alexa Fluor 555 Invitrogen 1:400
ED1 Millipore 1:250 Goat anti-mouse Alexa Fluor 488 Invitrogen 1:600
GFAP Millipore 1:400 Donkey anti-chicken Cy3 Millipore 1:500
Iba1 Wako 1:500 Goat anti-rabbit IgG Cy3 Linaris 1:300
PKM Abcam 1:500 Donkey anti-rabbit Alexa Fluor 555 Invitrogen 1:400
RBPMS Millipore 1:300 Goat anti-rabbit Alexa Fluor 488 Millipore 1:500
S100B Novus Biologicals 1:100 Goat anti-rabbit Alexa Fluor 488 Millipore 1:500
Tmem119 Synaptic System 1:500 Goat anti-mouse Alexa Fluor 488 Invitrogen 1:600
Longitudinal optic nerve sections
Cleaved caspase 3 Sigma-Aldrich 1:100 Donkey anti-rabbit Alexa Fluor 555 Invitrogen 1:400
ED1 Millipore 1:200 Goat anti-mouse Alexa Fluor 555 Invitrogen 1:500
GFAP Millipore 1:400 Donkey anti-chicken Cy3 Millipore 1:500
Iba1 Wako 1:400 Goat anti-rabbit Alexa Fluor 488 Invitrogen 1:500
S100B Novus Biologicals 1:100 Donkey anti-rabbit Alexa Fluor 555 Invitrogen 1:400
SMI-32 Biolegend 1:2000 Goat anti-mouse Alexa Fluor 555 Invitrogen 1:500
Tmem119 Synaptic System 1:500 Goat anti-mouse Alexa Fluor 488 Invitrogen 1:600