Fig. 4

CXCL13 knockdown alleviates PND-like cognitive deficits in mice. Mice were pretreated with CXCL13 shRNA or scrambled shRNA, subjected to surgery and then behavioral tests were performed 7 days after surgery. a Western blotting and densitometry of hippocampal CXCL13, as well as b RT-qPCR analysis of the corresponding mRNA. c Time to identify the target hole in training sessions and d latency time during memory phases were quantified in the Barnes maze. In the fear conditioning tests, freezing times were quantified e in context and f in response to a cue. All quantitative results (mean ± SD) shown were obtained with n = 8 animals per group. *P < 0.05 vs. control; ^#P < 0.05 vs. surgery. CXCL13 C-X-C motif chemokine 13, RT-qPCR reverse transcription-quantitative polymerase chain reaction, shRNA short hairpin RNA, sh-CRTL scrambled shRNA, sh-CXCL13 CXCL13 shRNA