Fig. 3

Pinocembrin treatment inhibits NLRP3 inflammation activation and enhances BNIP3-mediated mitophagy following IH exposures in mice. a The immunohistochemistry of NLRP3 in the hippocampus after exposure to IH treatment with or without pinocembrin. Bar = 100 μm. Western blots of NLRP3, caspase-1 p20, and ASC in the hippocampi from mice exposed to NA or IH, treating with vehicle or pinocembrin, respectively. b Western blots of NLRP3, caspase-1 p20, and ASC in the hippocampi from mice exposed to NA or IH, treating with vehicle or pinocembrin, respectively. c Representative pictures of double staining with BNIP3/IBA1 (white arrow) in hippocampi of mice obtained by a fluorescence microscope. Pinocembrin administration further increased the level of BNIP3 following IH exposures of hippocampi in mice. d Mice were exposed to NA or IH for 21 days and hippocampi were collected. Typical western blotting images of BNIP3/NIX, BNIP3, TOM20, and LC3 proteins of hippocampi in mice. ^#p < 0.01 vs. the control group; *p < 0.05 vs. the IH group; **p < 0.01 vs. the IH group