Fig. 6

Increased PVN activity during CSN stimulation. Eight to 10-week-old C57BL/6 mice were obtained, and the CSN was isolated and stimulated (600 μA, 10 Hz). Electrical activity recordings of the PVN of the hypothalamus were taken using a stereotaxic frame. Impact of CSN electrostimulation on PVN activity was evaluated. a A representative trace of activity in the PVN during CSN stimulation. b The average discharge was acquired per minute for baseline recordings and during stimulation. All individual traces or points represent one animal. The means are represented as ± SD. c After recording, the frontal plane of a representative brain was injected with dye to show the location of the recording electrode and a zoomed in image of the PVN. Both images shown are representative of the whole