Fig. 1

Examination of brain myeloid populations after a 60-min MCAO reveals a significant population of Tmem119⁺P2RY12⁺ cells with relatively higher MHC-II expression resides within the CD45^highCD11b⁺ gate. a Ipsilateral hemisphere from the sham brain (gated on live cells) myeloid populations shows that nearly all CD45^intCD11b⁺ cells are double-positive Tmem119⁺P2RY12⁺ while no significant subpopulation of Tmem119⁺P2RY12⁺ cells resides within the CD45^highCD11b⁺ gate. b Ipsilateral hemisphere from stroke brain shows that nearly all CD45^intCD11b⁺ cells are double-positive Tmem119⁺P2RY12⁺ and show a significant subpopulation of Tmem119⁺P2RY12⁺ cells within the CD45^highCD11b⁺ gate. c tSNE plots for the global examination of Live Tmem119⁺ pool in the sham brain. d tSNE plots for the global examination of Live Tmem119⁺ pool in the ischemic brain. The black arrow shows the CD45^high cluster which can be identified for other surface markers in MCAO tSNE plots. e Relative frequency of CD45^int population as a percentage of Live Tmem119⁺ cells is significantly lower after MCAO. f Relative frequency of CD45^high population as a percentage of Live Tmem119⁺ cells is significantly increased after MCAO. g Median fluorescence intensity (MFI) of CD45, as a measure of surface expression, in Live Tmem119⁺ cells is significantly increased after MCAO. h Surface expression of Tmem119 in Live Tmem119⁺ cells is significantly decreased after MCAO. i Surface expression of P2RY12 in Live Tmem119⁺ cells is significantly decreased after MCAO. j Surface expression of MHCII in CD45^int versus CD45^high subpopulations of CD11b⁺Tmem119⁺P2RY12⁺ cells shows significantly higher MHCII expression in the CD45^high subpopulation after MCAO. (n = 4/gp, unpaired student t test, **p < 0.01, ***p < 0.001, ****p < 0.0001)