Fig. 4

Mutant LRRK2 exacerbated Pg-induced peripheral IL-17A secretion and IL-17RA upregulation. The mice were equally divided into four groups, F + C, F + Pg, 1441 + C, and 1441 + Pg. a IL-17A protein levels in serum were examined using multiplex cytokine and chemokine analysis. n = 4–5, *P < 0.05. Two-way ANOVA and Tukey’s test were used for analysis. b Pg-treated mononuclear cells were stimulated for about 24 h and the IL-17A protein levels in the supernatant were measured by ELISA. n = 4, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Two-way ANOVA and Tukey’s test were used for analysis. c Representative images of western blot of IL-17RA obtained from SN tissue and quantitative analysis. n = 4, ***P < 0.001. Two-way ANOVA and Tukey’s test were used for analysis. d Representative images of co-localization of TH (green) and IL-17RA (red) from four groups, F + C, F + Pg, 1441 + C, and 1441 + Pg (scale bar = 20 μm)