Fig. 5

Blood-brain barrier breakdown after BCAS is exacerbated by Nrf2 deficiency. a There were no significant alterations in IgG levels in the corpus callosum. b There was a significant effect of surgery and genotype on IgG levels in the internal capsule, with Nrf2^−/− displaying highest density of IgG. (Double asterisk indicates post hoc significant difference between sham and BCAS Nrf2^−/− mice, double number sign and number sign indicate a difference between Nrf2^−/− BCAS and wild type or Nrf2^+/− BCAS groups respectively) c There was a significant increase in IgG levels in the optic tract with BCAS surgery (asterisk indicates post hoc significant difference between sham and BCAS Nrf2^−/− mice). Mean ± SEM. **p < 0.01, #p < 0.05, ##p < 0.01. d Representative images of IgG staining in the optic tract, brown stain depicts IgG, blue is haematoxylin counterstain. Scale bar 50 μm