Fig. 2

Leukocyte infiltration in the SC of HSV-2^esi latently infected mice. a Nissl staining of Mock (n = 4) and HSV-2^esi (n = 5) SCs from two independent experiments. Red arrowhead points to a cell accumulation spanning across laminae I and II in the dorsal horn and the gracile fasciculus, yellow arrowhead points to cell accumulations within the white matter, and magenta arrowhead points to a cell accumulation in lamina IX in the ventral horn. b Single-plane confocal images of Mock (n = 4) and HSV-2^esi (n = 5) SCs from two independent experiments. CD45 in green and NeuN in white. Red arrowhead points to a CD45^high cell accumulation in lamina I in the dorsal horn, yellow arrowhead points to a CD45^high cell accumulation within the white matter, and magenta arrowhead points to a CD45^high cell accumulation in the ventral horn. c Z-stack confocal images of an HSV-2^esi SC (lamina IX). CD45 in green and NeuN in white