Fig 5.
IL-1RA treatment reduces neutrophil recruitment to the injured spinal cord, but not IgG infiltration, 6 hours post-injury. Female C57BL/6 mice underwent a 70kD SCI and were treated with human IL-1RA (i.p. 100mg/kg, n=8) immediately following, and 5 hours after impact, or saline as control (n=4). Tissue was collected 6 hours post-injury and fixed spinal cord was stained for infiltrating neutrophils and IgG. Neutrophil cell density was calculated at the epicentre and 3 sections either side, corresponding to ±1.0mm (ai). Representative images of neutrophil density in the epicentre of spinal cord lesions with, and without IL-1RA treatment, scale bar represents 50μm (aii). Volume of infiltrating IgG was measured as a percent of volume of spinal cord (bi). Representative images of IgG staining 0.6mm from the lesion epicentre (bii). Data are presented as mean ±SEM. ***p<0.0001 in two-way ANOVA, ##p<0.01 with post-hoc test