Fig. 2

Transcriptional control of Nostrill by the NF-кB signaling pathway in murine microglia following LPS stimulation. a Effects of inhibition of the NF-кB signaling pathway on the induction of Nostrill in response to LPS stimulation. BV2 cells were pre-treated with the NF-кB inhibitors SC-514 (100 μM) or JSH-23 (30 μM) 1 h prior to LPS (10 μg/ml) stimulation for 6 h. DMSO was used for stimulation of control cells. b Effects of inhibition of NF- кB signaling on the expression of IL-1β, a NF-кB target gene. Data represent means ± SEM from three independent experiments. Expression levels were validated by real-time quantitative PCR. Gapdh was used as a reference gene for normalization. *p < 0.05, **p < 0.01, and ***p < 0.001 vs control. ††p < 0.01 between indicated groups