Fig. 5

The effects of L-NAME and/or PDI knockdown on the amounts of SNO-thiol and total thiol-PDI or -P2X7R, and PDI-P2X7R bindings following SE. As compared to vehicle (V), L-NAME (L) reduces the amount of SNO-thiol-PDI and PDI-P2X7R bindings without affecting PDI expression and the amount of total thiol-PDI under physiological condition. Thus, S-nitrosylation ratio of PDI is decreased. L-NAME abrogates SE-induced alterations in PDI-P2X7R bindings and S-nitrosylation ratio of PDI, except PDI expression. As compared to control siRNA (C), PDI siRNA (P) abolishes the changes in PDI-P2X7R bindings and S-nitrosylation ratio of P2X7R following SE, except its expression. a Representative Western blot for S-nitrosylation and thiolization on PDI and its expression. b Quantification of analyses of S-nitrosylation and thiolization on PDI and its expression. Open circles indicate each individual value. Horizontal bars indicate the mean value. Error bars indicate SEM (*^#p < 0.05 vs. control animals and vehicle, respectively; *p < 0.05 vs. vehicle; n = 7, respectively). c Representative Western blot for the PDI-P2X7R bindings following SE. d Quantification of analyses of PDI-P2X7R bindings. Open circles indicate each individual value. Horizontal bars indicate the mean value. Error bars indicate SEM (*^#p < 0.05 vs. control animals and vehicle or control siRNA, respectively; n = 7, respectively). e Representative Western blot for S-nitrosylation and thiolization on P2X7R and its expression. f Quantification of analyses of S-nitrosylation and thiolization on P2X7R and its expression. Open circles indicate each individual value. Horizontal bars indicate the mean value. Error bars indicate SEM (*^#p < 0.05 vs. control animals and control siRNA, respectively; n = 7, respectively)