Fig. 6

The effects of PDI knockdown on neuronal death, microglial activation, and astroglial apoptosis following SE. PDI siRNA inhibits microglial activation by inhibiting p65-S276 NF-κB phosphorylation and attenuates astroglial apoptosis in the dentate gyrus following SE, while it does not affect SE-induced neuronal death. a Representative photos for FJB-positive degenerating CA1 neurons following SE. b Quantification of analyses of the effect of PDI siRNA on SE-induced CA1 neuronal degeneration. Open circles indicate each individual value. Horizontal bars indicate the mean value. Error bars indicate SEM (n = 7, respectively). c Representative photos for p65-S276 NF-κB phosphorylation in microglia in the CA1 region (left panels) and TUNEL-positive astrocytes in the dentate gyrus (right panels) following SE. d-f Quantification of analyses of the effect of PDI siRNA on microglial activation (d), p65-S276 NF-κB phosphorylation in microglia (e), and astroglial apoptosis in the dentate gyrus (f). Open circles indicate each individual value. Horizontal bars indicate the mean value. Error bars indicate SEM (*^#p < 0.05 vs. control animals and control siRNA, respectively; n = 7, respectively)