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Table 1 Primers used in the qPCR analyses

From: Microglial inflammation after chronic spinal cord injury is enhanced by reactive astrocytes via the fibronectin/β1 integrin pathway

Gene Forward primer Reverse primer
Gapgh 5′-GACTTCAACAGCAACTCCCACTCT-3′ 5′-GGTTTCTTACTCCTTGGAGGCCAT-3′
Gfap 5′-TGTACTAACAGAGCGAGCCTATGC-3′ 5′-GGGACTTGCTGCCTTTAACATTGG-3′
Col1α1 5′-TGGTCACGTTCAGTTGGTCAAAGG-3′ 5′-AACCCTGGAAACAGACGAACAACC-3′
Gap43 5′-GACAGGATGAGGGTAAAGAAGACC-3′ 5′-AGAGAGAGAGGGCTCATAGGTAGG-3′
Tnf 5′-TGTGAAAACGGAGCTGAGCTGTCC-3′ 5′-GGTTCAGTGATGTAGCGACAGCCT-3′
Msr1 5′-TGGAACACATGAAGGACATGGAGG-3′ 5′-AATTCCCATGTTCCTGGACTGACG-3′
Mrc1 5′-ATGTGTCGTGATCGCAGAATTGTG-3′ 5′-GGTGTATAACATATCTCTGGGAGG-3′
Ccl1 5′-TGTTACAGAAAGATGGGCTCCTCC-3′ 5′-ACTGAGGGAAACTGCAGTCTCTGG-3′
Ccl8 5′-AGAAGCTGACTGGGCCAGATAAGG-3′ 5′-ACTCACTGACCCACTTCTGTGTGG-3′
Ccl17 5′-ATGTGAAGAAGGCCATCAGATTGG-3′ 5′-TTCGCCTGTAGTGCATAAGAGTCC-3′
Ccl22 5′-AAACTTCAGACTTCCTTGGCCTCC-3′ 5′-GAAATCTGATTCTGAGCCTGCTCC-3′
Ccl24 5′-GCAGAAACGGAGGATTTCAACAGC-3′ 5′-CTTCTTAGGCATGCACCATCATGC-3′
Cxcl1 5′-GGGAGGCTGTGTTTGTATGTCTTG-3′ 5′-CGAGACCAGGAGAAACAGGGTTAA-3′
Cxcl2 5′-CGGATGGCTTTCATGGAAGGAGTG-3′ 5′-GCTAAGCAAGGCACTGTGCCTTAC-3′
Cxcl3 5′-TAACAACTCCTGAGAGTTCATACC-3′ 5′-CCTTCAAGTTAAGAATAGGCTTGG-3′
Cxcl9 5′-GGAAGCAGTAAATTCTGCGAGTGG-3′ 5′-GCACAAAAACCACCTGCATGCAGF-3′
Cxcl10 5′-GTCCTGAGACAAAAGTAACTGCCG-3′ 5′-AACTTAGAACTGACGAGCCTGAGC-3′
Cxcl11 5′-CTGTGAATGAATGGTAGGGATGGC-3′ 5′-CTGTGAATGAATGGTAGGGATGGC-3′
Cxcl13 5′-ATACCCAACCCACATCCTTGTTCC-3′ 5′-CGCAAACCTCTTGTTAGTACGAGC-3′
Il1b 5′-GGGCTGGACTGTTTCTAATGCCTT-3′ 5′-CCATCAGAGGCAAGGAGGAAAACA-3′
Il6 5′-CCACAGTGAGGAATGTCCACAAAC-3′ 5′-GCTCTCCTAACAGATAAGCTGGAG-3′
Il10 5′-GCTCCAAGACCAAGGTGTCTACAA-3′ 5′-CCGTTAGCTAAGATCCCTGGATCA-3′
Il12b 5′-CAGAGCCAGGGAGCTAATGTATGC-3′ 5′-ATTTGCATAATAGGGCCTGGTCCC-3′
Il15 5′-GTCCAAATGTTCATCAACACGTCC-3′ 5′-TGATCCAAGTGGCTCATTATCTCC-3′
Il23a 5′-AGAATAAAGTCTCGAGCCCTTGGC-3′ 5′-TACAGATAATGGCCAAAGCCTGGG-3′
Tgfb1 5′-GTGACAGCAAAGATAACAAACTCC-3′ 5′-GAGCTGAAGCAATAGTTGGTATCC-3′
Arg1 5′-TGTATCCCAGCAGTTCCTTTCTGG-3′ 5′-ACCTCTCTGGATACCTTTGCTTCC-3′
Igf1 5′-AACTGTCTGGGCCTAAAAGCAAGC-3′ 5′-AACAGAGGAGACTTTGCTGTGAGC-3′
Chil3 5′-ATACACAGTGCAAGTTGCAAGGGC-3′ 5′-GCTGGTACAGCAGACAAGACATCC-3′
Pdgfa 5′-AGACAGATGTGAGGTGAGATGAGC-3′ 5′-ACGGAGGAGAACAAAGACCGCACG-3′
Pdgfb 5′-TACCTCCACTCTGTGTCTTCTTCC-3′ 5′-CATCCCATTACAACCTTGCTCACC-3′
Fcgr3 5′-CTCACAAACAAGATGCCTACTGCC-3′ 5′-GGATAACAGCTATGCCATCAACCC-3′
Fcgr2b 5′-GTCACTTCTGTGAGTCCTGAAACC-3′ 5′-TATAAGCAGTTCCCACGTTGCTGC-3′
CD86 5′-GAACCATCTCTAGATCCAAGAGCC-3′ 5′-TTGGCTGTCTTATCCTTGCACAGC-3′
CD163 5′-AGCTAAATGGAACAAGAGCCCAGG-3′ 5′-GACCCTATTGCGAACAAGCAAACC-3′
Fn1 5′-TTTACAGCTTCTCCAAGCATCGCC-3′ 5′-TCCCTATTGATCCCAGACCAAACC-3′
Fn1 transcript variant 1 5′-ACGGTTTCCCATTACGCCAT-3′ 5′-TCATCCGCTGGCCATTTTCT-3′
Fn1 transcript variant 2 5′-ATGAGAAGCCTGGATCCCCT-3′ 5′-GGAAGGGTAACCAGTTGGGG-3′
Fn1 transcript variant 3 5′-ATGAGAAGCCTGGATCCCCT-3′ 5′-TGTCCGCCTAAAGCCATGTT-3′
Fn1 transcript variant 4 5′-ACGGTTTCCCATTACGCCAT-3′ 5′-TCATCCGCTGGCCATTTTCT-3′
Fn1 transcript variant 5 5′-GACCCTTACACGGTTTCCCA-3′ 5′-TCATCCGCTGGCCATTTTCT-3′
Fn1 transcript variant 6 5′-ATGAGAAGCCTGGATCCCCT-3′ 5′-GAGAGCTTCCTGTCCTGTCT-3′
Fn1 transcript variant 7 5′-GACCCTTACACGGTTTCCCA-3′ 5′-TCATCCGCTGGCCATTTTCT-3′
Lamb2 5′-TCGTTCTCCTCATATGTGCCCTCC-3′ 5′-TTGAAAGCTCTTGCTAGCCAGGAG-3′