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Table 3 Antibodies used in flow cytometry

From: Chronic exposure to IL-6 induces a desensitized phenotype of the microglia

Target antigen   Format Dilution Cat Number Manufacturer
Fc blocker CD16/32 Purified 1:250 553142 BD Pharmingen
Primary antibodies CD11b APC-Cy7 1:400 557657 BD Pharmingen
CD45 PerCPCy5 1:400 557235 BD Biosciences
MHCII FITC 1:400 553623 BD Pharmingen
CD11c PE 1:400 557401 BD Pharmingen
Ly6C FITC 1:400 553104 BD Pharmingen
ICOSL PE 1:400 12-5985-82 BD Bioscience
CD86 PE-Cy7 1:400 560582 BD Pharmingen
CD80 APC 1:400 560016 BD Pharmingen
CD11c PE 1:400 557401 BD Pharmingen
F4/80 APC 1:400 17-4801-82 eBioscience
CD3e FITC 1:400 553062 BD Pharmingen
CD4 APC-Cy7 1:400 552051 BD Pharmingen
CD8 PerCP 1:400 553036 BD Pharmingen
T-bet PerCPCy5.5 1:400 45-5825 eBioscience
RORγt APC 1:400 17-6988-82 eBioscience
Foxp3 PECy7 1:400 25-5773-80 eBioscience
Gata3 PE 1:400 560074 BD Pharmingen