Chronic exposure to IL-6 induces a desensitized phenotype of the microglia

Table 3 Antibodies used in flow cytometry

Target antigen		Format	Dilution	Cat Number	Manufacturer
Fc blocker	CD16/32	Purified	1:250	553142	BD Pharmingen
Primary antibodies	CD11b	APC-Cy7	1:400	557657	BD Pharmingen
	CD45	PerCPCy5	1:400	557235	BD Biosciences
	MHCII	FITC	1:400	553623	BD Pharmingen
	CD11c	PE	1:400	557401	BD Pharmingen
	Ly6C	FITC	1:400	553104	BD Pharmingen
	ICOSL	PE	1:400	12-5985-82	BD Bioscience
	CD86	PE-Cy7	1:400	560582	BD Pharmingen
	CD80	APC	1:400	560016	BD Pharmingen
	CD11c	PE	1:400	557401	BD Pharmingen
	F4/80	APC	1:400	17-4801-82	eBioscience
	CD3e	FITC	1:400	553062	BD Pharmingen
	CD4	APC-Cy7	1:400	552051	BD Pharmingen
	CD8	PerCP	1:400	553036	BD Pharmingen
	T-bet	PerCPCy5.5	1:400	45-5825	eBioscience
	RORγt	APC	1:400	17-6988-82	eBioscience
	Foxp3	PECy7	1:400	25-5773-80	eBioscience
	Gata3	PE	1:400	560074	BD Pharmingen

ISSN: 1742-2094