Fig. 7

The effects of anti-miR-142 on the expression of FMRP, NF-κB p65 in rat hippocampus and neuron-like cells. a Representative image indicating FMRP and β-tubulin Ш immunopositive cells in rat hippocampus of the control, PTSD, sh-miR-NC, and sh-miR groups. b The relative level of FMRP in rat hippocampus of each group. Sample western blots for each protein are presented with β-actin as a loading control. c The relative level of NF-κB p65 expression in rat hippocampal nuclei of each group. Sample western blots for each protein are presented with PARN as a loading control for nuclear protein. d Representative image indicating NF-κB p65 and β-tubulin Ш immunopositive cells in rat hippocampus of each group. e The representative image of FMRP-positive neuron-like cells induced from PC12 cells in the CM, L-CM, and Sh-miR groups. The red arrow indicates GFP expression in neuron-like cells 2 days after transfection of LV-anti-miR-142 and the green arrows indicate PSD95 positive cells. f The relative level of FMRP in neuron-like cells in the CM, L-CM, Sh-miR-NC, and Sh-miR groups. Sample western blots for each protein are presented with β-actin as a loading control. g The relative level of NF-κB p65 in the nuclei of neuron-like cells of each group. Sample western blots for each protein are presented with PARN as a loading control for nuclear protein. n = 6 rats per genotype. Bar graphs represent the mean ± SEM of independent experimental triplicates. One-way or two-way ANOVA followed by Bonferroni’s post hoc test; *P < 0.05, **P < 0.01, ***P < 0.001