Fig. 2

Rotenone dose-dependently induces neurodegeneration and α-synuclein Ser129 phosphorylation in mice. a The representative images of Neu-N and PSD95 immunostaining in mice were shown. b–e Quantitative analysis of Neu-N⁺ cell number and PSD-95 immunostaining density in the dentate gyrus (DG) and cortex of mice. n = 5–6. f The representative blots of Neu-N and PSD-95 detected by Western blot were shown. g, h Quantitative analysis of Neu-N and PSD95 blots. n = 6. i The representative images of Ser129-phosphorylated α-synuclein immunostaining in the hippocampus and cortex of mice were shown. j Quantitative analysis of the density of Ser129-phosphorylated α-synuclein immunostaining. n = 4. *p < 0.05, **p < 0.01; Scale bar = 200 and 50 μm in A and I, respectively