Skip to main content

Table 2 List of primary and secondary antibodies used for immunofluorescence staining

From: Suppression of TLR4-MyD88 signaling pathway attenuated chronic mechanical pain in a rat model of endometriosis

Antibody Host Company Catalog ID Dilution Incubation conditions
HMGB1 Rabbit Cell Signaling Technology #6893 1:200 Overnight 4 °C
MyD88 Rabbit Abcam ab2064 1:400 Overnight 4 °C
TLR4 Rabbit Invitrogen 48-2300 1:400 Overnight 4 °C
TRPV1 Guinea pig Abcam ab10295 1:400 Overnight 4 °C
PGP9.5 Guinea pig Abcam ab10410 1:400 Overnight 4 °C
pNF-κB-p65 Rabbit Abcam ab86299 1:400 Overnight 4 °C
GFAP Chichen Abcam ab4674 1:1000 Overnight 4 °C
CGRP Goat Abcam ab36001 1:3000 Overnight 4 °C
IBA1 Goat Abcam ab5076 1:500 Overnight 4 °C
NeuN Mouse Abcam ab104224 1:1000 Overnight 4 °C
Anti-rabbit IgG Alexa Fluor 488 Donkey Jackson ImmunoResearch 711-545-152 1:400 1 h RT
Anti-Guinea pig IgG Alexa Fluor 594 Donkey Jackson ImmunoResearch 706-585-148 1:400 1 h RT
Isolectin GS-IB4 Alexa Fluor™ 594 Conjugate   Invitrogen I21413 1:400 1 h RT
Anti-goat IgG Alexa Fluor 488 Donkey Abcam ab150129 1:400 1 h RT
Anti-goat IgG Alexa Fluor 594 Donkey Jackson ImmunoResearch 705-585-147 1:400 1 h RT
\