Suppression of TLR4-MyD88 signaling pathway attenuated chronic mechanical pain in a rat model of endometriosis

Table 2 List of primary and secondary antibodies used for immunofluorescence staining

Antibody	Host	Company	Catalog ID	Dilution	Incubation conditions
HMGB1	Rabbit	Cell Signaling Technology	#6893	1:200	Overnight 4 °C
MyD88	Rabbit	Abcam	ab2064	1:400	Overnight 4 °C
TLR4	Rabbit	Invitrogen	48-2300	1:400	Overnight 4 °C
TRPV1	Guinea pig	Abcam	ab10295	1:400	Overnight 4 °C
PGP9.5	Guinea pig	Abcam	ab10410	1:400	Overnight 4 °C
pNF-κB-p65	Rabbit	Abcam	ab86299	1:400	Overnight 4 °C
GFAP	Chichen	Abcam	ab4674	1:1000	Overnight 4 °C
CGRP	Goat	Abcam	ab36001	1:3000	Overnight 4 °C
IBA1	Goat	Abcam	ab5076	1:500	Overnight 4 °C
NeuN	Mouse	Abcam	ab104224	1:1000	Overnight 4 °C
Anti-rabbit IgG Alexa Fluor 488	Donkey	Jackson ImmunoResearch	711-545-152	1:400	1 h RT
Anti-Guinea pig IgG Alexa Fluor 594	Donkey	Jackson ImmunoResearch	706-585-148	1:400	1 h RT
Isolectin GS-IB4 Alexa Fluor™ 594 Conjugate		Invitrogen	I21413	1:400	1 h RT
Anti-goat IgG Alexa Fluor 488	Donkey	Abcam	ab150129	1:400	1 h RT
Anti-goat IgG Alexa Fluor 594	Donkey	Jackson ImmunoResearch	705-585-147	1:400	1 h RT

ISSN: 1742-2094